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| Registros recuperados: 40 | |
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| Tayale, Alexandre; Gueguen, Yannick; Treguier, Cathy; Le Grand, Jacqueline; Cochennec-laureau, Nathalie; Montagnani, Caroline; Ky, Chin-long. |
| Producing high quality cultured black pearls from Pinctada margaritifera is one of the major challenges for the "pearl oyster" industry in French Polynesia. In order to assess donor effect on cultured pearl quality, wild Pinctada margaritifera originating from the Tuamotu Archipelago were used in a duplicated grafting experiment. After 12 months of culture, nucleus retention was assessed and seven pearl quality traits recorded on the 454 cultured pearls harvested from the experiment. The traits scored were nacre thickness and pearl weight, surface defects, lustre, grade, and the colour components: 1) darkness of cultured pearl colour, and 2) visual perception of colour class (bodycolor and/or overtone). Our results demonstrate for the first time that... |
| Tipo: Text |
Palavras-chave: Cultured pearl oyster; Pearl quality; Nacre; Donor mollusc; Genetic selection; Pteriidae; Blacklip pearl oyster; Pinctada margaritifera; French Polynesia. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00116/22757/20541.pdf |
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| Gueguen, Yannick; Montagnani, Caroline; Joubert, Caroline; Marie, Benjamin; Belliard, Corinne; Tayale, Alexandre; Fievet, Julie; Levy, Peva; Piquemal, David; Marin, Frederic; Le Moullac, Gilles; Ky, Chin-long; Garen, Pierre; Lo, Cedrick; Saulnier, Denis. |
| Tahiti’s pearl farming industry plays a major socio-economic role in French Polynesia. In an increasingly competitive market where the production of high quality pearls becomes essential, research can help secure and ensure sustainable production. In that context, Ifremer, in close collaboration with the “direction des resources marines” (French Polynesian government agency) has developed research projects on the “sustainable development of pearl farming”. This program is organized along 3 axes: (1) understanding the animal physiology and initiating a genetically selective breeding program of donor oysters; (2) understanding pearl oyster larvae dispersal and recruitment; (3) understanding the molecular mechanisms underlying biomineralization processes... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Biomineralization; Pinctada margaritifera. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00167/27840/26049.pdf |
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| Ky, Chin-long; Barre, Philippe; Noirot, Michel. |
| The objective of the paper was to identify the number of major loci explaining caffeine content in coffee seeds. Investigations were based on previously published results: (1) Caffeine binds to chlorogenic acids in a 1:1 molecular ratio; (2) Between species, the caffeine content is correlated to the chlorogenic acid content; (3) Only a part of chlorogenic acids is bound to caffeine. Especially, the content ratio between caffeine and chlorogenic acids varied between species. For identifying the number of major loci, a quantitative trait locus (QTL) approach was carried out using an interspecific cross between two highly differentiated species-Coffea liberica dewevrei and Coffea pseudozanguebariae, the latter being a caffeine-free species. As main finding,... |
| Tipo: Text |
Palavras-chave: Coffea; Chlorogenic acids; Caffeine; QTL; Interspecific cross. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00152/26298/24429.pdf |
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| Ky, Chin-long; Blay, Carole; Sham-koua, Manaarii; Vanaa, Vincent; Lo, Cedrik; Cabral, Philippe. |
| Individual Pinctada margaritifera molluscs were collected from the Takapoto atoll (Tuamotu Archipelago, French Polynesia) and used to produce ten first generation full-sib families in a hatchery system, following artificial breeding protocols. After three years of culture, these progenies were transferred to Rangiroa atoll (Tuamotu Archipelago, French Polynesia) and tested for their potential as graft donors. A large-scale grafting experiment of 1500 grafts was conducted, in which a single professional grafter used ten individual donor oysters from each of the ten families, grafting 15 recipient oysters from each donor. The recipient oysters were all obtained from wild spat collection in Ahe (Tuamotu Archipelago, French Polynesia). After 18 months of... |
| Tipo: Text |
Palavras-chave: Cultured pearl oyster; Pearl quality; Progeny effects; Heritability; Genetic selection; Pinctada margaritifera; French Polynesia. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00152/26310/24380.pdf |
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| Joubert, Caroline; Linard, Clementine; Le Moullac, Gilles; Soyez, Claude; Saulnier, Denis; Teaniniuraitemoana, Vaihiti; Ky, Chin-long; Gueguen, Yannick. |
| In this study, we analyzed the combined effect of microalgal concentration and temperature on the shell growth of the bivalve Pinctada margaritifera and the molecular mechanisms underlying this biomineralization process. Shell growth was measured after two months of rearing in experimental conditions, using calcein staining of the calcified structures. Molecular mechanisms were studied though the expression of 11 genes encoding proteins implicated in the biomineralization process, which was assessed in the mantle. We showed that shell growth is influenced by both microalgal concentration and temperature, and that these environmental factors also regulate the expression of most of the genes studied. Gene expression measurement of shell matrix protein... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00210/32095/30535.pdf |
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| Ky, Chin-long; Blay, Carole; Sham-koua, Manaarii; Lo, Cédrik; Cabral, Philippe. |
| The top aquaculture species in French Polynesia is Pinctada margaritifera, a mollusc grown for the production of a unique gem: the black pearl. One of the challenges facing the pearl farming industry is to "produce less but better pearls" through genetic improvement. An experimental hatchery system was used to generate full-sib families to be tested for their potential as donor "oysters". A large-scale grafting experiments was done and seven cultured pearl quality traits: grade, surface defects, lustre, darkness level, visual colour categories, circles and shape categories were recorded. Our results revealed, for the first time, significant phenotypic relationships between these quality traits. The grade A cultured pearl class had the largest proportion of... |
| Tipo: Text |
Palavras-chave: Pearl "oyster"; Pinctada margaritifera; Donor "oyster"; Cultured pearl quality; Pearl grade; Pearl colour; Pearl shape; Selective breeding. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00190/30130/28592.pdf |
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| Ky, Chin-long; Demmer, Jonathan; Sham-koua, Manaarii; Cabral, Philippe. |
| Production of larger, rounder, high-quality cultured pearls with fewer circles is one of the main challenges of Pinctada margaritifera aquaculture faced by every pearl farm in French Polynesia. Although bigger pearl sizes can be achieved through surgreffe operations (implantation of a second nucleus following pearl harvest), control of the development of pearl circles and shapes still remains unclear, as illustrated by grafter’s empirical rules, where often the surgreffe process is only performed after production of uncircled and round pearl shapes. The present study was designed with a real pearl by pearl traceability to reveal for the first time the development of circles and shapes from graft and surgreffe, in relation to the size of the pearl sac. This... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Pinctada margaritifera; Surgreffe; Circled pearls; Pearl shapes; Pearl sac. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00276/38690/37187.pdf |
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| Ky, Chin-long; Lau, Christophe; Sham Koua, Manaarii; Lo, Cedrik. |
| The black-lipped pearl oyster Pinctada margaritifera is of high economic importance in French Polynesia. Arecent decline in pearl value led to the initiation of several genetic breeding programs aiming to improve production traits, including oyster shell growth, which dictates the time of grafting, size of the implanted nuclei, and biomineralization capacities. We assessed shell diameters on hatchery-produced spat to analyze juvenile growth performance of four half-sib families derived from polyandry (one dam crossed with two sires) and polygyny (two dams crossed with one sire) using gametes obtained by thermal shock or gonad scarification. Spat growth was monitored over 3 mo, with shell diameter measured weekly. Results revealed that the spawning method... |
| Tipo: Text |
Palavras-chave: Pearl oysters; Pinctada margaritifera; Juvenile; Growth performance; Spawning procedure. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00310/42167/41470.pdf |
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| Ky, Chin-long; Nakasai, Seiji; Molinari, Nicolas; Devaux, Dominique. |
| Cultured pearls quality produced by the mollusc Pinctada margaritifera var. cumingi is determined by a panel of criteria, of which shape is one of the most relevant. Cultured pearls with round and semi-round (RS) shapes are the most valuable. Decreasing the proportion of other pearl shapes (OT: baroque, button, drop, oval), and especially the circled pearls (CL) and rejects (rebuts: RT), is an important challenge for the pearl industry. The present study examined the effect of grafter skill and season of graft on the variation in proportions of CL, RS, OT and RT cultured pearl shape categories in a mono-site culture (Mangareva island lagoon). Six large-scale grafting experiments, carried out in the same way as commercial grafting sessions, were made by... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; Cultured pearl; Grafter skill; Pearl shapes; Circled pearls; Grafting season. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00216/32745/31150.pdf |
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| Molinari, Nicolas; Abou-badra, Mathieu; Marin, Gregory; Ky, Chin-long; Amador, Noemi; Gamez, Anne Sophie; Vachier, Isabelle; Bourdin, Arnaud. |
| Background: COPD is largely under-diagnosed and once diagnosed usually at a late stage. Early diagnosis is thoroughly recommended but most attempts failed as the disease is marginally known and screening marginally accepted. It is a rare cause of concern in primary care and spirometry is not very common. Exhaled carbon monoxide (eCO) is a 5-seconds easy-to-use device dedicated to monitor cigarette smoke consumption. We aimed to assess whether systematic eCO measurement in primary care is a useful tool to improve acceptance for early COPD diagnosis. Methods: This was a two-center randomized controlled trial enrolling 410 patients between March and May, 2013. Whatever was the reason of attendance to the clinic, all adults were proposed to measure eCO during... |
| Tipo: Text |
Palavras-chave: COPD; Screening; Exhaled CO; Smoking; Behaviour. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00270/38142/36280.pdf |
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| Ky, Chin-long; Okura, Retsu; Nakasai, Seiji; Devaux, Dominique. |
| In French Polynesia, the aquaculture of Pinctada margaritifera (Linnaeus, 1758) covers a large maritime exploitation area, spread over nearly 20 degrees latitude and longitude, with numerous pearl farms located in three archipelagos (Gambier, Society, and Tuamotu). As these archipelagos have specific seasonal temperature patterns each year, pearl oysters are subject to disparate and contrasting environmental regimes. This study aimed to examine the specificity of commercial pearl quality traits (n ¼ 2,236 samples) at the archipelago scale, in such a way as to provide preliminary data to design the most appropriate strategy for the distribution of hatchery-produced phenotypes. A large and standardized grafting experiment using the same donor phenotype was... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Pinctada margaritifera; Large-scale geographic effects; Pearl color; Pearl grade; Pearl shape; Pearl size. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00357/46798/46665.pdf |
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| Latchere, Oihana; Fievet, Julie; Lo, Cedrik; Schneider, Denis; Dieu, Stephanie; Cabral, Philippe; Belliard, Corinne; Ky, Chin-long; Gueguen, Yannick; Saulnier, Denis. |
| The present study investigated the effect of electrolysis on the biomineralization capacities of juveniles of the mollusk Pinctada margaritifera for the first time. Size-selected individuals from two groups, “Medium” and “Large”, from a multi-parental family produced in a hatchery system were subjected to electrolysis under a low voltage current over a nine-week experimental period. The growth of the juveniles was individually monitored and assessed weekly by wet weight and shell height measurements. At the end of the experiment, mantle tissue was sampled for biomineralization-related gene expression analysis. Electrolysis significantly increased pearl oyster growth in terms of shell height and wet weight for Large juveniles from the 5th and the 2nd week,... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Pinctada margaritifera; Electrolysis; Biomineralization; Gene expression. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00343/45410/44924.pdf |
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| Le Pabic, Lore; Parrad, Sophie; Sham Koua, Manaarii; Nakasai, Seiji; Saulnier, Denis; Devaux, Dominique; Ky, Chin-long. |
| Size is the most important and valuable quality of the cultured black-lip pearl, Pinctada margaritifera. As this pearl aquaculture is carried out at numerous grow-out sites, this study analyzes the environmental influence on pearl size parameters (nacre weight and thickness) in relation to the recipient oyster biometric parameters (shell thickness, height, width, and oyster weight) at harvest time. Toward this end, an experimental graft was designed by using a homogeneous donor oyster phenotype. The recipient oysters were randomly and equally transferred and reared in five commercial and contrasting grow-out locations. Overall inter-site comparisons revealed that the cultured pearl size (N=2168) and the biometric parameters of the recipient oysters were... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; Cultured pearl size; Donor oysters; Recipient oysters; Gene expression; Environmental influences. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00321/43191/42738.pdf |
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| Ky, Chin-long; Le Pabic, Lore; Koua, Manaarii Sham; Molinari, Nicolas; Nakasai, Seiji; Devaux, Dominique. |
| The black-lipped pearl oyster, Pinctada margaritifera is the most important farmed mollusc species in French Polynesia. Donor oyster selection among wild P. margaritifera individuals, chosen according to their inner shell colour, makes it possible to obtain the broadest range of cultured pearl colours of any species. This study demonstrates the relative influence of using black [B] or red [R] outer shell phenotypes, combined with green [G] or yellow [Y] inner shell phenotypes, on pearl darkness level, colour categories and lustre. A large scale grafting experiment was designed and carried out over five grow-out locations, covering three archipelagos: Tuamotus, Society and Gambier. Results revealed that the [B + G] phenotypes may be used as donors to... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Pinctada margaritifera; Shell colour phenotype; Pearl colour; Environment. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00285/39637/38129.pdf |
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| Ky, Chin-long; Le Moullac, Gilles. |
| Size is the most important and valuable quality trait of cultured pearls produced by the black-lipped pearl oyster, Pinctada margaritifera. In French Polynesia, several breeding programmes have been started that aim to improve this size trait, which is highly related to shell growth rate in both recipient and donor oysters. Shell growth rate dictates the time of grafting, size of implanted nuclei and bio-mineralisation potential of the mantle and pearl sac. We assessed shell growth rate through routine digital shell biometric analysis on 22 hatchery families produced between 2005 and 2008. These included full-sib families and half-sib families derived from polyandry (one dam crossed with two or more sires). Results showed that: 1) a significant family... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; Pearl oyster; Hatchery-produced families; Growth performance; Shell diameter; Cultured pearl size. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00387/49810/50384.pdf |
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| Blay, Carole; Planes, Serge; Ky, Chin-long. |
| Grafting associates two distinct genotypes, each of which maintains its own genetic identity throughout the life of the grafted organism. Grafting technology is well documented in the plant kingdom, but much less so in animals. The pearl oyster, Pinctada margaritifera, produces valuable pearls as a result of the biomineralisation process of a mantle graft from a donor inserted together with a nucleus into the gonad of a recipient oyster. To explore the respective roles of donor and recipient in pearl formation, a uniform experimental graft was designed using donor and recipient oysters monitored for their growth traits. At the same time, phenotypic parameters corresponding to pearl size and quality traits were recorded. Phenotypic interaction analysis... |
| Tipo: Text |
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| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00387/49809/50382.pdf |
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| Ky, Chin-long; Demmer, Jonathan; Blay, Carole; Lo, Cedrik. |
| Pinctada margaritifera is an economically important marine bivalve species for cultured pearl production in French Polynesian aquaculture. In order to evaluate the influence of donor oyster age on pearl quality traits, experiments were conducted over 6 years using both grafts and surgreffe operations. At harvest, 6 pearl quality traits were recorded and compared: surface defects, luster, grade, darkness level, and visual color. Analyzing the quality traits of pearls harvested in the initial graft process and those of pearls obtained from surgreffe experiments allowed a comparison of the influence of pearl sac cells originating from the initial mantle graft, which aged together with their recipient oysters. The results demonstrated a significant decrease... |
| Tipo: Text |
Palavras-chave: Pearl oyster age; Pinctada margaritifera; Surgreffe; Pearl grade; Pearl colour. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00283/39472/37953.pdf |
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| Ky, Chin-long; Blay, Carole; Aiho, Vaite; Cabral, Philippe; Le Moullac, Gilles; Lo, Cedrik. |
| In French Polynesia, the aquaculture of P. margaritifera is carried out in numerous grow-out sites, located over three archipelagos (Gambier, Society and Tuamotu). To evaluate the impact of macro-geographical effects of these growing sites on pearl quality traits, five hatcheries produced families were used as homogeneous donor oysters in an experimental graft. The molluscs were then reared in two commercial locations: Tahaa island (Society) and Rangiroa atoll (Tuamotu). At harvest, eight pearl quality traits were recorded and compared: surface defects, lustre, grade, circles, shape categories, darkness level, body and secondary colour and visual colour categories. Overall inter-site comparison revealed that: 1) all traits were affected by grow-out... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; Cultured pearl quality; Grow-out location; Environment. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00276/38687/37186.pdf |
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| Registros recuperados: 40 | |
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